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ENHANCEMENT SOLUTION FOR ENHANCING CHEMILUMINESCENCE AND METHOD FOR PREPARING CHEMILUMINESCENT SOLUTION
ENHANCEMENT SOLUTION FOR ENHANCING CHEMILUMINESCENCE AND METHOD FOR PREPARING CHEMILUMINESCENT SOLUTION
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机译:用于增强化学发光的增强溶液和用于制备化学发光溶液的方法
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摘要
Disclosed are an enhancement solution for enhancing chemiluminescence, and a method for preparing a chemiluminescent solution. The method comprises Step A: dissolving methylglucamine in water, adjusting the pH to 8-9, then adding an N-alkyldimethylmannosamine quaternary ammonium salt, acridine orange, BSA, glycine, MgCl2, and ZnCl2 respectively to a diethanolamine solution, stirring to dissolve them, and diluting to a constant volume, to obtain an enhancement solution; and Step B: diluting a chemiluminescent substrate with the enhancement solution, to obtain a chemiluminescent solution. When used in the field of immunoassays, the enhancement solution of the present invention increases the sensitivity and linear range of detection, reduces the cost, and is free of contamination.
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