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Nanoliter qPCR platform for parallel quantitative assessment of reductive dehalogenase genes
Nanoliter qPCR platform for parallel quantitative assessment of reductive dehalogenase genes
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机译:纳升qPCR平台,用于并行定量评估还原性脱卤素酶基因
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摘要
Combinations of reductive dehalogenase (rdh) genes are a distinguishing genomic feature of closely-related organohalogen-respiring bacteria. This feature can be used to deconvolute the population structure of organohalogen-respiring bacteria in complex environments and to identify relevant subpopulations, which is important for tracking interspecies dynamics needed for successful site remediation. The present disclosure encompasses embodiments of a nanoliter qPCR platform to identify organohalogen-respiring bacteria by quantitatively identifying major orthologous reductive dehalogenase gene groups.
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