首页>
外国专利>
Method for cancer targeting treatment and detection of arginine using albumin-binding arginine deiminase fusion protein
Method for cancer targeting treatment and detection of arginine using albumin-binding arginine deiminase fusion protein
展开▼
机译:用白蛋白结合的精氨酸脱亚氨酶融合蛋白进行癌症靶向治疗和检测精氨酸的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention provides a pharmaceutical composition containing albumin-binding arginine deiminase (AAD) fusion protein for treating cancer or other arginine-dependent diseases. The AAD fusion protein can be purified from both soluble and insoluble fractions of crude proteins, binds to human serum albumin (HSA) or animal serum albumin and has its high activity with longer half life for efficient depletion of arginine in cancer cells. The specific activities of wild-type ADI and AAD fusion protein in the present invention are about 20 and about 19 U/mg (at physiological pH 7.4), respectively. The composition can be used alone or in combination with at least one chemotherapeutic agent to give a synergistic effect on cancer treatment and/or inhibiting metastasis. The AAD fusion protein can also be used as a component for detection and quantitative analysis of arginine in a testing kit for various samples including blood, food and analytical samples.
展开▼