Method of making three-dimensional, leaf-based scaffold for three-dimensional cell culture

摘要

The method of making a three-dimensional, leaf-based scaffold for three-dimensional cell cultures includes washing a quantity of Ficus religiosa leaves, then treating the washed Ficus religiosa leaves in a sodium hydroxide solution to obtain alkali-treated Ficus religiosa leaves. The alkali-treated Ficus religiosa leaves are washed, and then superficial tissue is removed from the alkali-treated Ficus religiosa leaves to obtain Ficus religiosa leaf skeletons. The Ficus religiosa leaf skeletons are dried and then consecutively immersed in distilled water, a phosphate buffer saline solution, and plain Dulbecco's modified Eagle's medium (DMEM) to form the three-dimensional scaffolds for three-dimensional cell cultures. Each three-dimensional scaffold can be used for growing three-dimensional cell cultures, such as human mesenchymal stem cell cultures.