complex transgenic trace locus in a plant, plant or seed, method for producing in a plant a complex transgenic trace locus and expression construct

摘要

complex transgenic trace locus in a plant, plant or seed, method for producing in a plant a complex transgenic trace locus and expression construct The present invention relates to methods for producing in a plant a complex transgenic trace locus comprising At least two altered target sequences in a genomic region of interest The methods involve the use of two or more double strand break inducing agents, each of which may cause a double strand break in a target sequence in the genomic strand. interest that results in a change in the target sequence. Complex transgenic trait loci are also presented in plants. A complex transgenic trace locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Also presented are plants, plant cells, plant parts and seeds comprising one or more complex transgenic trait loci.