Method for quantifying multiple specific immunoglobulins of antigen and to calibrate an appropriate device, test system multialergeno, and kit of parts.

摘要

Method for quantifying multiple specific immunoglobulins of antigen and to calibrate an appropriate device, test system multialergeno, and kit of parts, the invention provides a method to quantify multiple immunoglobulins specific antigen in a sample to test the methods. ODO comprising using a dilution series of anti immunoglobulin antibodies.Fragments or derivatives thereof, immobilized in a solid support in combination with a serial dilution of a reference sample of immunoglobulin to generate multiple curves of binding capacity.Such curves of binding capacity are combined with specific dose-response curves generated for each specific antigen to be tested, using serum samples of known reactivity to these antigens to provide a calibration system that allows more precise analysis of the IMU Noglobulina specific antigen in a sample.The invention also relates to methods for calibrating a device suitable for testing multiple specific immunoglobulins that bind antigen in the multiple antigens, or its fragments immobilized on a solid support. Also provided is a system and multialu00e9rgenos test kits for use in methods.