Expression vector, uses vector expression, method for transfection of eukaryotic cells, use of the method, the use of the cell, methods of antibody production and transfection method.

摘要

Herein is reported an expression vector comprisingan antibody light chain expression cassette,an antibody heavy chain expression cassette, anda selection marker expression cassette, wherein the expression cassettes are arranged unidirectional, and wherein the expression cassettes are arranged in the 5 to 3 sequence of antibody heavy chain expression cassette, antibody light chain expression cassette and selection marker expression cassette. Further are reported herein methods for the generation of antibody producing cells and the use of these cells for the recombinant production of antibodies.