Method for obtaining crystals of a basic amino acid hydrochloride comprising generating a basic amino acid using microbial cells by fermentation in a fermentation broth or by an enzymatic method in a solution of reaction of enzyme using the cells as catalyst.

摘要

Method for obtaining crystals of a basic amino acid hydrochloride from a fermentation broth amino acid or basic solution of enzyme reaction ".It is an object of this invention to provide a method to separate and obtain a basic amino acid hydrochloride from a fermentation broth amino acid or basic solution of enzyme reaction, the enzyme reaction was catalysed by viable cells of a microbe produt Or a basic amino acid, each containing sulfate ions, by which method produces the product.And the qualities are almost the same and are guaranteed more easily in comparison with the conventional technique, a method that includes the steps of: (1) adding the chloride of a metal selected from the group consisting of calcium, potassium, magnesium and barium, the fermentation broth A basic amino acid or the solution of the enzyme reaction containing the tones of sulphate,To precipitate the tones of sulfate as crystals of the resulting metal sulfate, (2) removing the crystals of metal sulfate solution of basic amino acid, (3) to cool the fermentation broth amino acid or basic solution of enzyme reaction, in which the crystals of sulphate of meta L are removed.While maintaining the concentration of sulfate metal below its saturated concentration to precipitate basic amino acid as crystals of hydrochloride, and (4) separating the crystals of hydrochloride of amino acid basic for collecting them.