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Isolation and History of Mu_____ mu__mRNA from Mu__

机译:Mu__的Mu_____ mu__mRNA的分离和历史

摘要

The invention provides a reliable method for recovering RNA samples from biological tissues with permanently immobilized formalin, paraffin wax. It includes 5 (a) submission of samples in the form of alpha P1, P1, P1, P, and so on.B) Contact with the above sample solution, including the approximate 10 K concentration of the active protease and the heating of the sample at delta_ lambda_ _mu A in a temperature range of about 30 to 80 or so, for a period of about 12~20 hours.C) Added a concentration of the solution to the approximate ten of the active proteases from 10 (b) for a period of time of about 5 to about 30 minutes in one representative Ross temperature was recovered from about 40 to about 75 degrees C, and (d) the above RNA from the solution. The additional assimilation stage C) greatly improved the recovery of RNA.
机译:本发明提供了一种用永久固定的福尔马林,石蜡从生物组织中回收RNA样品的可靠方法。它包括5(a)以alpha P1,P1,P1,P等形式提交的样品。B)与上述样品溶液接触,包括大约10 K浓度的活性蛋白酶和加热样品在约30至80左右的温度范围内,约12至20小时的时间。C)将溶液的浓度添加到10(b)中约10种活性蛋白酶中在一个代表性的罗斯温度中,从约40至约75℃回收约5至约30分钟的时间,并且(d)从溶液中回收上述RNA。额外的同化阶段C)大大提高了RNA的回收率。

著录项

  • 公开/公告号CY1115586T1

    专利类型

  • 公开/公告日2017-01-04

    原文格式PDF

  • 申请/专利权人 PANGAEA BIOTECH S.A.;

    申请/专利号CY200911000760T

  • 发明设计人 ROSELL COSTA RAFAEL;TARÓN ROCA MIGUEL;

    申请日2009-07-16

  • 分类号C12N15/10;C12Q1/68;

  • 国家 CY

  • 入库时间 2022-08-21 13:39:38

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