SYSTEM FOR CAPTURING AND MODIFYING LARGE PIECES OF GENOMIC DNA AND CONSTRUCTING ORGANISMS WITH SYNTHETIC CHLOROPLASTS,

摘要

pThe functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes./p[WO2009045550A2]