Bidirectional separation of peptides and/or amino acids by the TLC technique

摘要

the allocation of the peptides and / or amino acids is that for the chromatography with silica gel modified adsorbentu layer based on chemically binding organic lead 2 - directional development of chromatogram mixtures of peptides and / or amino acids with use but in one direction the mobile phase containing lower alcohol or a mixture of lower alcohols and water.the proportion by volume 1: 1 to 99: 1 and organic acids: acetic, formic, propionic and / or their salts used singly or their mixture concentration in the l - 500 mmol / dm3 and the composition of mobile phase used to drive development in the second direction contains mixed given alcohol alone and water in the proportion by volume 1: 99 - 1: 1 and formic acids:,trifluorooctowy, pentafluoropropionowy, heptafluoromasu0142owy, trichlorocetic acid and / or their salts used singly or their mixture, the concentration in the l - 500 mmol / dm3.