Method for purification of the recombinant Exotoxin A, recombinant Exotoxin A muteins and the recombinant proteins containing fragments of Exotoxin A

摘要

the object of the application is a protein purification involving egzotoksynu0119 and, muteinu0119 egzotoksyny and or fragment, comprising: (a) dissolving the sample containing the protein in buffer solubilizuju0105cym at alkaline ph and low concentrations of urea.and (b) reduction in the concentration of urea and ph by dilution using dilution buffer and / or by dialysis to dilution buffer before further purification of protein. the object of the application is also a protein conjugate or composition obtained this way.the application also relates to the treatment of the human or animal comprising administration of recombinant protein conjugate or compositions and treatment of human or animal body comprising administration of proteins or compositions received way of notification.