Method for producing stable active substance concentration gradient in the cell culture microsystems

摘要

process for the production of a stable concentration gradient of the active substance in mikrosystemach to conduct cell culture, characterized by that in the first stage tanks fluid supply chamber mikrourzu0105dzenia fills with solvents, sequentially: first and tanks consecutive chambers (blank and the solvent suspension cell culture)and the last chamber (chamber indicator) solvent coloured indicator substance in order to tanks on one side mikrourzu0105dzenia introduces liquid and leans mikrosystem, so to force its flow from those supplying the chamber mikrourzu0105dzenia to zbi orniku00f3w powerful located opposite to its side, and after filling the compartmentsthe liquid tank is removed.in the second stage is the suspension cells to at least two supply tanks located on one side mikrourzu0105dzenia (blank and each breeding competent) and solvent coloured indicator substance to the last tank (chamber indicator) and he s it mikrosystem,so to force fluid flow from the supply chamber to the tanks by tanks situated opposite to their side, so that the level of fluid in the opposite tanks up. in the third stage is the short-term culture of cells for their adhesion to surface compartments.in the fourth stage, empty tanks on both sides of the chambers, the chambers are filled with fluid and is a measured volume of solvent to the first chamber supply cell suspension (blank),solution of the active substance to the second and subsequent supply chambers (breeding competent) and coloured solution supply tank unit to last chamber (chamber indicator), then leans mikrosystem angle from 10 to 90 degrees in the direction of empty tank s,forcing a fluid flow chamber by completely emptying tanks supply pre filled fluid, however, without emptying the content, after which removes liquid from all tanks.in the fifth stage is inserted in the side tanks wypu0142ywowej referred to, a small volume of an appropriate solvent, according to the composition of liquids in each of the chambers and is mikrosystem direction empty tanks to force fluid flow in the mikrourz u0105dzenia and completely emptying tanks supply,then mikrosystem tilts in the opposite direction and force the fluid return flow chambers, the alternating function pochylania mikrourzu0105dzenia is repeated several times until the desired distribution of the concentration of coloured indicator chamber, after which the to the liquid tank.in the sixth stage tanks with equal volume fluid proportional to the composition mixture to exhaust ports of adjacent chambers to them - with an appropriate solvent, dye solution or solution of the active substance. in the seventh stage mikrourzu0105dzenia tanks shall be protected to ensure stability of concentration gradients.