Method for producing proteins in Pichia pastoris that lack detectable cross-binding activity to antibodies against host cell antigens

摘要

A method of producing a recombinant glycoprotein in Pichia pastoris that lacks cross-binding activity detectable with antibodies prepared against host cell antigens, comprising: (a) providing a recombinant Pichia pastoris host cell that does not exhibit any β-activity mannosyltransferase 1, 2, 3 and 4 with respect to an N-glycan or O-glycan, where the ß-mannosyltransferase genes 1, 2 and 3 have undergone deletion or disruption, including the host cell a nucleic acid molecule encoding the recombinant glycoprotein (b) culturing the host cell in a medium under conditions effective to express the recombinant glycoprotein; and (c) recovering the recombinant glycoprotein from the medium to produce the recombinant glycoprotein lacking detectable cross-binding activity with antibodies prepared against host cell antigens.