Specific activation of a regulatory T cell and its use for the treatment of asthma, allergic diseases, autoimmune diseases, graft rejection and tolerance induction

摘要

A method for determining whether a substance can activate a regulatory T cell (Treg) by interaction with the HIV-1 gp120 binding site of CD4, comprising: (a) preselecting a substance that can interact with the site of gp120 binding of HIV-1 from CD4, (b) providing a solution comprising a Treg cell, (c) adding a preselected substance according to (a) under conditions that allow the substance to interact with a Treg cell, (d ) measuring whether a Treg cell has been activated, in which an activated Treg cell identifies the substance as a Treg cell activator, in which step (a) of claim 1 comprises: i) providing a first solution comprising CD4 , ii) provide a second solution comprising CD4, iii) add to the first solution a substance to be tested and HIV-1 gp120 under conditions that allow the binding of HIV-1 gp120 with CD4, iv) add to the second dissol The gp120 of HIV-1 under the conditions of (c) that allow the binding of the gp120 of HIV-1 with CD4, v) measure in the first and second solution if the gp120 of HIV-1 has joined CD4, in which a reduced amount of HIV-1 gp120 bound in the first solution indicates that the substance can interact with the gp120 binding site of HIV-1 gp120 of CD4.