Method for culturing Lawsonia intracellularis bacteria in persistently infected McCoy cells

摘要

A method of obtaining McCoy cells persistently infected with Lawsonia intracellularis bacteria, comprising: - infecting McCoy cells with Lawsonia intracellularis bacteria, - culturing infected McCoy cells in a suitable medium at an oxygen concentration of less than 18% to reach a culture of McCoy cells infected with Lawsonia intracellularis bacteria, - passing at least part of said culture to fresh medium, and - without adding uninfected McCoy cells to the medium, culturing infected McCoy cells contained in said medium at least part in the fresh medium at an oxygen concentration of less than 18% to obtain persistently infected McCoy cells.