Peripheral diagnostic procedures for Alzheimer's disease screening using beta amyloid and intercellular communication

摘要

In vitro diagnostic procedure for Alzheimer's disease, the procedure comprising: (a) using two samples of fibroblast skin cells from the subject; (b) treating one of the cell samples with a beta amyloid peptide (Aβ); (c) culturing each of the cell samples, the one treated with Aβ and the untreated one, in a culture medium; (d) obtain images of each of the cell samples, the one treated with Aß and the untreated one, to obtain at least one image of each sample; (e) analyze the at least one image of each sample by at least one morphological analysis procedure with respect to a control known to have Alzheimer's disease (AD); and (f) in which, if both the Aβ treated and untreated cell sample have an AD type phenotype, the diagnosis is positive for AD and, if the Aß treated cell sample has an AD type phenotype and the untreated cell sample does not, the diagnosis is negative for AD.