Procedure for the ex-vivo study of the initial response of different cell types in atherosclerosis (Machine-translation by Google Translate, not legally binding)

摘要

Procedure for the ex-vivo study of the initial response of different cell types in atherosclerosis. The novelty of this model lies in the incubation of the cells of interest with the complete secretoma of the atheroma plaques to evaluate the cellular response. This incubation allows a more direct study of the cellular response to factors secreted by atheromatous plaques, instead of using isolated factors (vegf, sdf-1). This procedure would allow the evaluation of strategies to modulate the cellular response in response to atherosclerotic damage, for example in the case of endothelial progenitor cells (ecps), to promote vascular remodeling in therapies based on the use of these cells, although it can equally be applied to any cell type associated with atherosclerosis. It also allows to evaluate the effect of drugs in this initial framework of cellular response to atherosclerosis. (Machine-translation by Google Translate, not legally binding)