Gene expression system using alternative splicing in insects

摘要

A method for expressing a functional protein in an insect by an alternative splicing specific sex of an RNA transcript, the method comprising: a) transcribing, in said insect, a heterologous polynucleotide from an expression system for providing an RNA transcript, the expression system comprising: a promoter operably linked to a heterologous polynucleotide sequence encoding the functional protein, defined between an initiation codon and a stop codon; and a control sequence and intron splice cutting comprising a binding domain protein; wherein the binding domain proteins comprising the consensus sequence of DNA shown in SEQ ID NO: 1 or its RNA equivalent, wherein the control sequence splicing intron splice comprises a sequence of donor cutting GT splice at its 5 'end (5'-GT), and wherein the control sequence intron splice is flanked by a nucleotide guanine (G) 5'; and wherein the control sequence YY splicing intron was 3'to the ATG start codon of the heterologous polynucleotide sequence; b) alternatively, subjecting splicing the RNA transcript of the heterologous polynucleotide sequence, in cooperation with a spliceosome, to produce a first messenger RNA spliced ​​(mRNA), comprising a frame continuous open reading extending from the initiation codon to the stop codon, and another product of further alternative spliced ​​mRNA comprising a continuous open reading frame extending from the initiation codon to termination codon, defining said functional protein for expression.