Methods for the production of a cosmetic composition from a hatching fluid from Salmonidae eggs and uses thereof to improve the cosmetic appearance of the skin

摘要

A method to reduce or prevent the cosmetic appearance or predominance of wrinkles, fine lines, hyperpigmentation, laxity, dry skin, scale formation and / or loss of transepidermal water on the skin of a mammalian animal, which comprises administering a cosmetic composition to said animal, wherein said composition is obtained or can be obtained with a method comprising at least the steps of: a) suspending Salmonidae eggs in a minimum volume of water, preferably salmon eggs; b) induce rapid, synchronized hatching of said eggs, preferably so that the hatching is completed in less than 2 hours for more than 95% of the embryos; c) filter the hatched eggs to obtain hatching fluid; and d) filtering the hatching fluid to obtain the composition, wherein the step of filtering the hatching fluid comprises at least the steps of: (i) filtering the hatching fluid using a filter with a pore size of at least 5 μm, and collect the filtrate; (ii) filter the filtrate from step (i) using a filter with a pore size of 0.30-0.60 µm, and collect the filtrate; (iii) exchanging the water in the filtrate of step (ii) with a pharmaceutically acceptable buffer by performing diafiltration using a filter with an exclusion size of less than 15 kDa; (iv) optionally repeat the diafiltration stage of stage (iii); (v) optionally diluting the solution of step (iii) or (iv) so that the solution has an enzymatic activity of 3000-5000 mU / l, measured by the solution's ability to cleave the Factor Xa chromogenic substrate ( CH3OCO-D-CHA-Gly-Arg-pNA-AcOH); (vi) filter the solution from step (iii), (iv), or (v) using a filter with a pore size of 0.15-0.30 μm, and collect the filtrate; and (vii) preparing said cosmetic composition from the filtrate of step (vi).