Method for the rapid determination of the susceptibility or resistance of bacteria to antibiotics

摘要

A method for rapidly evaluate the susceptibility of a strain of bacteria isolated to an antibiotic which inhibits the synthesis of cell wall comprising: a) preparing a bacterial culture from a bacterial strain isolated; b) combining one or more doses of an antibiotic that inhibits cell wall with different parts of the bacterial culture, in which the concentration of each of the one or more doses correlates with thresholds antibiotic resistance for the strain bacteria isolated antibiotic which inhibits the synthesis of the cell wall; c) incubation of the bacterial culture and one or more doses of antibiotic; d) evaluating cell length or cell size of the bacteria incubated for each of the one or more doses of antibiotic that inhibits cell wall synthesis; e) classifying the susceptibility of the bacterial strain isolated antibiotic which inhibits the synthesis of the cell wall based on cell lengths or cell sizes of the bacteria associated with each dose of the one or more doses of antibiotic that inhibits synthesis of the cell wall, wherein a dose comprises a minimum concentration of antibiotic that inhibits the synthesis of cell wall determined empirically, which results in cell enlargement or increase in cell size in strains of the bacteria which are sensitive to the antibiotic which inhibits the synthesis of the cell wall, and wherein said minimum concentration of antibiotic that inhibits the synthesis of the cell wall is lower than the minimum inhibitory concentration (MIC) of the bacteria strain to antibiotic inhibits the synthesis of the cell wall to indicate susceptibility.