Differentiation of human embryonic stem cells with pancreatic endocrine lineage

摘要

A method for producing pancreatic hormone producing cells from pluripotent stem cells, comprising the steps of: a. culture of pluripotent stem cells, b. differentiate pluripotent stem cells into cells that express characteristic markers of the definitive endodermal lineage, c. differentiate cells that express characteristic markers of the definitive endodermal lineage into cells that express characteristic markers of the pancreatic endodermal lineage, and d. differentiate the cells that express characteristic markers of the pancreatic endodermal lineage into cells that express characteristic markers of the pancreatic endocrine lineage, in which the expression of MAFA in cells that express characteristic markers of the pancreatic endocrine lineage is increased by a method comprising the stages of cultivating cells expressing characteristic markers of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin dependent kinase inhibitor to cause an increase in MAFA expression.