DETECTION AND ENUMERATION OF LEGIONELLA PNEUMOPHILA

摘要

A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms:(1) contacting said microorganisms of said sample with repair compounds and a growth medium, and (2) incubating the product of step (1),and (3) detecting and enumerating said microorganisms, in which the microorganisms are of the species Legionella pneumophila, and in which the repair compounds comprise:(a)serine;(b)threonine;(c)a compound containing calcium ions at a dose of 10-6 to 10-2 mM;(d)a compound containing magnesium ions at a dose of 10-6 to 10-2 mM;(e)a compound containing potassium ions;(f) glutamic acid or a salt thereof; and (g)pyruvic acid or a salt thereof. The invention also discloses a kit for detecting and enumerating viable microorganisms of the species Legionella pneumophila in a sample suspected of containing said microorganisms.