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RT-QPCR ANALYSIS OF MICRO-DISSECTED MATERIAL FROM STAINED FFPET SECTION
RT-QPCR ANALYSIS OF MICRO-DISSECTED MATERIAL FROM STAINED FFPET SECTION
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机译:固定FFPET切片中的微解剖材料的RT-QPCR分析
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摘要
The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70°C for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method.
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