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METHODS AND COMPOSITIONS FOR MODULATING PRFA MEDIATED VIRULENCE GENE ACTIVATION IN LISTERIA SPP.
METHODS AND COMPOSITIONS FOR MODULATING PRFA MEDIATED VIRULENCE GENE ACTIVATION IN LISTERIA SPP.
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机译:调制在莉斯特利亚SPP中PRFA介导的毒力基因激活的方法和成分。
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摘要
Methods of assessing expression of a PrfA mediated expression construct in a Listerial host cell are provided. Aspects of the methods include maintaining a Listerial host cell that includes a PrfA mediated expression construct in a defined medium containing a reducing agent and evaluating expression of a product from the expression construct to assess expression of the PrfA mediated expression construct. Also provided are methods of producing and using a Listerial vaccine, as well as defined media for use in such methods.
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