METHOD FOR PRODUCING OF HYALURONIDASE CONJUGATE WITH POLYETHYLENE PIPERAZINE DERIVATIVES AND APPLICATION OF OBTAINED CONJUGATE

摘要

The invention relates to a method for producing immobilised enzymatic preparations, in particular, to production and use of a new active enzyme conjugate with a polymer carrier. The conjugate is characterized by the properties of the drug Longidaza and inhibits connective tissue hyperplasia; it also demonstrates our anti-inflammatory effect and can be used for manufacturing stable, active and safe-to-use sustained-release therapeutic preparations in the form of a suppository, ointment, injection or cosmetic cream, as well as for producing veterinary therapeutic drugs. The method comprises conjugating hyaluronidase with a water-soluble copolymer by methods involving carbodiimides or azides. The conjugation technique employs a copolymer of N-oxide 1,4-ethylene piperazine, (N-carboxymethylmethyl)-1,4-ethylene piperazinium or a hydrazide thereof, and 1,4-ethylene piperazine with the general formula (I), wherein n represents 40% to 90% of the total number of the monomer units, m represents 3% to 40% of the total number of the monomer units, and n+m+1=100%.