PLURIPOTENT STEM CELL EXPANSION AND PASSAGE USING A STIRRED TANK BIOREACTOR

摘要

Provided herein are novelmethods for expansion and passaging ofcell aggregates comprising stem cellsand/or differentiated cells and comprisingthe use of closed systems in stirred tankbioreactors. The methods of the inventionpermit closed system serial passage expansionof pluripotent stem cells and/or progenythereof with associated pluripotencymarkers and differentiation potential.