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Methods of co-detecting mRNA and small non-coding RNA
Methods of co-detecting mRNA and small non-coding RNA
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机译:共同检测mRNA和小的非编码RNA的方法
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摘要
Disclosed herein are methods of co-detecting presence of target messenger RNA (mRNA) and small non-coding RNA (for example, miRNA) in a sample. The disclosed methods can be used to simultaneously detect mRNA and small non- coding RNA in a single assay (for example in the same reaction or the same well of a multi-well assay). The methods can include contacting a sample with a plurality of nuclease protection probes including at least one probe which specifically binds to a target mRNA and at least one probe which specifically binds to a target small non-coding RNA, contacting the sample with a nuclease specific for single- stranded nucleic acids, and detecting the NPP, for example on a microarray.
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