METHOD FOR THE ULTRA-SENSITIVE DETERMINATION OF CATECHOLAMINES AND THEIR METABOLITES

摘要

The present invention relates to methods for the ultra-sensitive determination of one or more catecholamine(s) and metabolites thereof in a biological sample, comprising steps of phenylboronic acid (PBA) complexation, solid phase extraction (SPE) on hydrophilic-lipophilic-balanced (HLB) sorbents, liquid chromatography (LC) on pentafluorophenyl (PFP) columns, and tandem mass spectrometry (MS/MS), optionally using summation of multiple reaction monitoring (MRM) data.