GENOME EDITING BY CRISPR-CAS9 IN FILAMENTOUS FUNGAL HOST CELLS

摘要

The present invention relates to methods for modifying at least one target sequence in the genome of a filamentous fungal host cell by employing a Class II Cas9 enzyme, e.g., the S.pyogenes Cas9, together with a suitable guide RNA for each target sequence to generate a site-specific cut or nick in at least one genome target sequence, followed by the repair of the cut(s) and/or nick(s) via integration of one or more modified modified donor part of the filamentous fungal host cell genome through double recombination on each side of the nick(s).