Surface modified micropate menufacturing method for the surface modified micropate and immunoassays using the surface modified micropate

摘要

The present invention provides a surface modification method and a diagnostic plate capable of fixing Escherichia coli, which is generally distributed with a chemical species having a negative charge, with high efficiency. In addition, a diagnostic plate using the diagnostic plate according to the present invention is provided by demonstrating a diagnostic plate having a positive charge on its surface and an Immunoassay using E. coli genetically engineered to perform an antigen-antibody reaction.A diagnostic plate capable of binding to surface-expressing E. coli according to the present invention comprises: a substrate; A parylene-H thin film formed on one surface of the substrate; And a thin film formed on the parylene-H film and having positive charge function groups at both ends. The diagnostic method using the surface-modified diagnostic plate according to the present invention comprises: a substrate; a parylene-H thin film formed on one side of the substrate; and a thin film containing surface formed on the parylene-Forming a modified diagnostic plate; Immobilizing a specific protein surface expression E-coli on the surface-modified diagnostic plate; Reacting the antibody on the E-coli-fixed plate; And reacting the antigen with a plate on which the specific antibody has been reacted.;