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METHOD FOR OBTAINING MASS SPECTRUM OF IONS GENERATED AT CONSTANT TEMPERATURE BY MEASURING TOTAL ION COUNT AND USE OF MATRIX FOR QUANTITATIVE ANALYSIS USING MALDI MASS SPECTROMETRY
METHOD FOR OBTAINING MASS SPECTRUM OF IONS GENERATED AT CONSTANT TEMPERATURE BY MEASURING TOTAL ION COUNT AND USE OF MATRIX FOR QUANTITATIVE ANALYSIS USING MALDI MASS SPECTROMETRY
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机译:通过测量总离子数并利用马尔代质谱法定量分析矩阵来获取恒定温度下产生的离子质谱的方法
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摘要
The present invention relates to the use of the matrix for quantitative analysis using a MALDI mass spectrometry method, and by measuring the total ion count to obtain the mass spectrum of ions in the generated reproducible at a constant temperature. More particularly, the present invention is characterized in that it comprises the step of the sample is selected from among a plurality of mass spectra obtained from ions formed by applying energy to the mixed specimen, to the total ion count only the same mass spectrum each other , to a method for measuring the mass spectra of ions produced at a constant temperature. The present invention is a matrix with the sample to measure the equilibrium constant for the proton exchange reaction between, MALDI quantitative method to obtain the black line (calibration curve) for, and a MALDI mass spectrometry method Determination of sample analysis using a at a constant temperature, and It is for the purpose of the matrix for quantitative analysis using a MALDI mass spectrometry.
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