Disgregating device of biological material and corresponding manufacturing method and method for the preparation of cell suspensions and tissue micrografts

摘要

Separation device and a corresponding manufacturing method of a biological sample and the cell suspension and prepare tissue micro-graft method of A disgregating device (10) for a biological sample comprising: - a hollow outer body (11) forming an inner chamber (12); - an upper loading chamber 12a having a plurality of fine holes 13a with sharp edges and being easy to load a living sample MB to be degraded and a lower loading chamber 12b for easily collecting the living sample MB ' A fixed disintegration grid (13) housed transversely in the inner chamber (12) to form a collection chamber (12b); And - supplying a biological sample (MB) contained in the upper loading chamber (12a) into contact with the fine holes (13a) of the decomposition grid (13) as the blade rotor (14) rotating in the inner chamber Which is easy to cooperate with the fixed disintegration grid 13 and therefore causes degradation of the biological sample MB 'while the biological sample MB passes through the fine holes 13a, 14). The micropores 13a of the dissolution grid have a dimension or diameter comprised between 70 and 80 microns and are made of a metal sheet N of stainless steel so as to have a jagged sharp edge with several, Punch type molding using a conical or polygonal molding punch (P) to draw and tear a die, or a source of laser light again inside a sphere of a die-punch type molding process Is obtained by a die-punch type molding process used. By means of the special features and configuration of the micropores 13a, as confirmed by the experimental test, it is possible, without the aid of a chemical reagent, by the decomposing device 10 to provide the whole unaltered characteristic It is possible to prepare cell suspensions and tissue micrografts that retain viability, function, and viability, and thus the degradation device can be advantageously used in many medical, veterinary and cosmetic applications.