Method for increasing the proliferation and stemness of limbal stem cells

摘要

The present invention relates to a method for promoting the proliferation and stem cell function of a limbal stem cell by treating a wnt inhibitor to a limbal-derived epithelial cell culture medium. When the human limb-derived epithelial cell plate is cultured using the method according to the present invention, the proliferation of the limbal stem cells is enhanced by inhibiting the Wnt /? -Catenin signaling and the stem cell function of the limbal stem cells is enhanced, Cells can be obtained with high efficiency, and the success rate of transplantation of the limbal epithelial cell plate can be increased to the limbal stem cell deficient patient.