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METHOD FOR THE BODY-FLUID-BASED NEURODEGENERATIVE DISEASE DIAGNOSIS USING HIGH SENSITIVE IMMUNOASSAY OF AGGREGATED PROTEINS BY PHOTOOXIDATION-INDUCED AMPLIFICATION
METHOD FOR THE BODY-FLUID-BASED NEURODEGENERATIVE DISEASE DIAGNOSIS USING HIGH SENSITIVE IMMUNOASSAY OF AGGREGATED PROTEINS BY PHOTOOXIDATION-INDUCED AMPLIFICATION
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机译:光氧化诱导扩增对融合蛋白进行高灵敏度免疫分析的基于体液的神经退行性疾病诊断方法
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摘要
Discloses a method for diagnosing degenerative neurodegenerative diseases based on body fluids through high sensitivity photooxidative amplification immunoassay of aggregated proteins. A method for diagnosing a neurodegenerative disease based on body fluids through a highly sensitive photooxidative amplification immunoassay of a coagulase protein according to an embodiment of the present invention comprises the steps of preparing a body fluid sample, preparing a body fluid sample containing an antibody-Preparing a reference sample containing the unbound antibody labeling enzyme; Performing an enzymatic-substrate reaction on a body fluid sample and a reference sample, performing a photo-oxidation amplification process by continuously exposing light to the body fluid sample and the reference sample after the enzyme-substrate reaction, And indexing the photooxidative amplification pattern with time, and comparing the photooxidative amplification index of the body fluid sample with the reference sample to analyze the degenerative nervous system disease by analyzing the content of the coagulated protein in the body fluid The present invention relates to a method for detecting an aggregation protein in an oligomer or a monomer form by coagulation of an oligomer or a monomer in the form of an oligomer or monomer, And the amount of normal or abnormal protein aggregation is measured on the side By an effect that can accurately diagnose the neurodegenerative disease.
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