Method for screening yeast strains with enhanced recombinant protein secretion using yeast variants having cell-wall defect

摘要

The present invention relates to a method for producing a method for screening a yeast strain that secretes the target protein, a method of improving the secretion of the desired protein, the desired protein of the manufacturing method and the Gas1 or the secretion of its fusion protein enhanced yeast mutants introduced. The cell wall stress conditions a protein of interest produced by using a GAS1 deficient strain growth is inhibited in a yeast strain expressing a fusion protein coupled to the N- terminus of the Gas1 protein according to the invention, the cell walls formed in accordance with the secretion of the fusion protein since by confirming the increase in growth performance can determine effectively yeast strains secretion is increased, it may be useful to screen for enhanced strain secretion of a series of mutant libraries prepared by random mutagenesis for the mass production of the recombinant protein. ;