KS 513 a novel compound KS 513 isolated from the extract of Pseudolysimachion rotundum var. subintegrum and the composition comprising the same as an active ingredient for preventing or treating allergy disease inflammatory disease asthma or chronic obstructive pulmonary disease

摘要

The present invention relates to a novel compound isolated from an acidic extract of Pseudomonas sp., An extract of (3R, 5S, 5aS, 6R, 7S, 8R, 8aS) -8-chloro-8a-hydroxy- (3R, 5S, 5aS, 6R, 7S, 6S, 6S, 6R, 6S) 8R, 8aS) -8-chloro-8a-hydroxy-5- (2-hydroxy acetoxy) hexahydro-1H-3,6-methanocyclopenta [e] [1,3] dioxepin-7-yl 3,4-dihydroxybenzoate} Inflammatory, asthma or chronic obstructive pulmonary disease, which comprises, as an active ingredient, an anti-inflammatory agent (KS-513) as an active ingredient. Test of cell viability in RAW264.7 cells (Experimental Example 1) for the compounds according to the present invention; Effects of iNOS and COX-2, the precursor inflammatory mediator genes that induce gene expression in RAW 264.7 cells treated with LPS, and IL-1β, IL-6, and TNF-α mRNA expression levels of precursor inflammation-mediated cytokine genes Experiment (Experimental Example 2); Inhibition experiment on protein expression and NO production concentration of enzyme gene iNOS associated with NO production (Experimental Example 3); Inhibition experiment on the protein expression and PGE ₂ production concentration of the precursor inflammatory mediator gene COX-2 (Experimental Example 4); Inhibition of production of NO and PGE ₂ induced by LPS in RAW 264.7 cells, inhibition of iNOS and COX-2 protein by RAE 264.7 cells, inhibition of proinflammatory cytokines TNF-α, IL-1β and IL- And the expression of the gene and suppresses the secretion amount of TNF-α, IL-1β and IL-6, which are proinflammatory mediators of the proinflammatory cytokines, and the composition is used for the prevention and treatment of inflammation, asthma or chronic obstructive pulmonary disease A pharmaceutical composition, a health functional food or a health supplement.