METHOD FOR EVALUATING TOXICITY OF CHEMICAL USING ALGA

摘要

The present invention relates to a method for freezing and freezing algae cells by (a) thawing frozen algae cells by thawing, (d) thawing the resulting cell suspension by adding a medium to the suspension, and (b) (C) recovering part of the algae cells after the recovery culture step (b), diluting the medium with the medium, measuring the delayed luminescence of the algae cells, and using the obtained luminescence amount as the initial value data (D) an exposing step of mixing the algae cells and the test substance-containing solution after the confirming step (c) to prepare an exposed sample and culturing the exposed sample; (e) a step of exposing the exposed sample after the exposing step (F) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance on the basis of the evaluation value; A method for evaluating the toxicity of a chemical substance using an algae comprising the same.;