The probe system for real time quantitative and qualitative analyzing of biomaterial the reaction chamber with said probe system and the analyzing method thereof

摘要

The present invention relates to a probe system, which is contained in a reaction container having a light-permeable flat bottom surface and which is contained in a reaction container for receiving a sample therein, comprising a sequence complementary to a target nucleic acid sequence to be detected, A target probe-reporter probe connector comprising a target probe comprising a quencher, a reporter probe coupled to an end of the target probe and comprising a sequence that is non-complementary to the target nucleic acid sequence; And a capture probe which is contained in the bio chip formed on the upper surface of the bottom of the reaction vessel and includes a complementary sequence capable of hybridizing with the non-complementary sequence of the reporter probe, and a second fluorophore and a second quencher A probe system for real-time quantitative and qualitative analysis of biomaterials, a reaction vessel having the probe system, and an assay method using the probe system.