Composition for RT-qPCR analysis according to different pig breeds and method for assessing the expression levels of genes of interest using the same

摘要

The present invention relates to a composition comprising a standard expression gene used in quantitative RT-PCR analysis of pig breeds, and a method for quantifying the expression level of a target gene using the same. More particularly, the present invention relates to a pig gene PPIA, TBP, HSPCB, RPL4 (QRT-PCR) analysis kit for pork varieties comprising at least one reference gene selected from the group consisting of RPS18 and TOP2B standard expression genes, kit, standard expression gene selection And a method for quantifying a target gene expression amount of a pig using the same. By utilizing the present invention, it is possible to measure the precise expression amount of a target gene of each pig breed.