Method for producing nontoxic bee venom peptide

摘要

The present invention relates to a method for producing refined bee venom peptide in which allergic components are removed and refined bee venom in which allergic components are removed by being manufactured by the method. The method comprises the following steps: (a) adding a buffer solution to bee venom and diluting the same; (b) adding protease to a diluted solution diluted in the step (a) and performing enzyme reaction; (c) adding cooled ethanol to a reactant after enzyme reaction in the step (b) and coagulating protein; (d) separating the reactant coagulated in the step (c) by centrifugation and depositing coagulation; and (e) obtaining supernatant from the reactant in which the coagulation in the step (d) is deposited, and drying the same.