Use of the intermediates of the tricarboxylic acid cycle (TCA) to control the formation of ammonia in the cell culture

摘要

The present invention is directed to a method of reducing the ammonium ion concentration in a mammalian cell culture, a method of maintaining or increasing cell productivity in a mammalian cell culture, a method of maintaining or increasing cell growth in a mammalian cell culture, and a method of reducing the influence of ammonium ion accumulation on antibody glycosylation patterns in a cell culture, comprising steps of growing the cells in a cell culture medium, and contacting or administering an effective amount of a TCA intermediate composition to the cell culture medium.