A method of evaluating the reproductive productivity of pigs

摘要

FIELD: biotechnology.SUBSTANCE: from including the isolation of DNA, amplification of a fragment of the LIF gene using primers 5'-ATGTGGATGTGGCCTACGG-3' and 5'GGGAACAAGGTGGTGATGG-3', restriction of the amplified fragment of the LIF gene by the restriction endonuclease DraIII, genotyping and selection of animals with the AA/LIF genotype, the size of 407 bp. Corresponds to the AA genotype, two fragments of 266 and 144 bp in size. - the genotype of BB, three fragments of 407, 266 and 144 NP. - genotype AB. Animals with the AA/LIF genotype have high fertility rates.EFFECT: invention makes it possible assess more effectively to the fertility of sows of landrace and large white sows and select pigs genetically predisposed to higher fertility rates.2 dwg, 2 tbl, 2 ex