METHOD FOR TREATING MALIGNANT NEW FORMATIONS USING MAGNETIC HYPERTHERMIA AND PHARMACEUTICAL COMPOSITIONS FOR APPLICATION IN THE SPECIFIED METHOD

摘要

FIELD: medicine.SUBSTANCE: application of Zn-substituted magnetic nanoparticles based on manganese ferrite of the formula ZnMnFeO(x=0-0.9), with average size of less than 40 nm, the Curie temperature ranges from 39 to 550°C, and the coercive force is 5 to 250 Oe. A pharmaceutical composition comprising these Zn-substituted magnetic nanoparticles and a pharmaceutically acceptable carrier is also provided. A method for malignant neoplasms treatment with magnetic hyperthermia, including the following steps (i) and (ii), is also proposed. Step (i) includes magnetic nanoparticles delivery to the tumour region in an effective concentration, selected from the group of magnetic metal oxides including Zn-substituted magnetic nanoparticles based on manganese ferrite of the formula ZnMnFeO(x=0-0.9), with average size of less than 40 nm, the Curie temperature ranges from 39 to 550°C, and the average value of coercive force is 5 to 250 Oe. The step includes (ii) tumour region exposure to an alternating current (AC) magnetic field with an amplitude of 14 to 300 Oe and a frequency of 80 to 1000 kHz, where the exposure time of the said magnetic field at a temperature of 38 to 51°C is between 15 and 60 minutes. In addition, a method for experimental tumour cells damage by magnetic hyperthermia is proposed, comprising the following steps (i), (ii), (iii). At that, step (i) includes provision of magnetic nanoparticles selected from the group of magnetic metal oxides including Zn-substituted magnetic nanoparticles based on manganese ferrite of the formula ZnMnFeO(x=0-0.9), with average size of less than 40 nm, the Curie temperature ranges from 39 to 550°C, and the average value of coercive force is from 5 to 250 Oe. Step (ii) includes addition of the said magnetic nanoparticles to the cell culture. Step (iii) comprises exposure of the said cell culture to a magnetic field of PT with an amplitude of 14 to 300°C and frequency of 80 to 1000 kHz, where the exposure time of the said magnetic field at a temperature of 38 to 51°C is between 15 and 60 minutes.EFFECT: increased effectiveness of treatment due to uniform heating of the tumour tissue with reduced undesirable phenomena as a result of concomitant or subsequent selective damage or at least partial destruction of tumour cells in the tumour region without damaging the healthy surrounding tissue.36 cl, 10 dwg, 4 tbl, 7 ex