RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I FOR PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I, Pichia pastoris YEAST STRAIN - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I AND METHOD OF PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I

摘要

FIELD: genetic engineering.;SUBSTANCE: to increase yield of mature human apolipoprotein A-I it is produced by enzymatic hydrolysis of SUMO-specific protease SP2 of recombinant chimeric protein SUMO3-apoA-I, synthesised by Pichia pastoris yeast strain. Said strain is constructed by transformation of Pichia pastoris X-33 yeast strain with linearised plasmid DNA pPsecSUMO3-apoA-I. Recombinant chimeric protein SUMO3-apoA-I includes from N-terminal amino acid sequence of ubiquitin-like protein of human SUMO3, fused at C-terminal with amino acid sequence of mature human apoA-I protein.;EFFECT: invention can be used for producing mature human apolipoprotein A-I.;3 cl, 1 tbl, 5 dwg, 7 ex