SETS OF OLIGONUCLEOTIDE PROBES AND METHODS OF GASTROINTESTINAL MICROBIOTA PROFILING

摘要

FIELD: biotechnology.;SUBSTANCE: group of inventions relates to biotechnology and is represented by a set of oligonucleotide probes for profiling of gastrointestinal microbiota (GI), where mentioned set comprises in particular (a) oligonucleotide consisting of (ai) nucleotide sequence selected from ACGCTTGCACCCT (SEQ ID NO: 1) optionally with no more than three substituted bases or AGGGTGCAAGCGT (SEQ ID NO: 27) optionally with no more than three substituted bases, and (aii) 0-5 nucleotides in addition to (ai), where oligonucleotide according to (a) is able to be hybridized with SEQ ID NO: 27 or SEQ ID NO: 1, respectively, in stringent hybridization conditions. Methods are also presented for profiling of patient's GI microbiota and methods for diagnosing or monitoring patient's disease or condition or prediction or assessment of risk of patient's disease or condition, in which oligonucleotide probes set is used.;EFFECT: using inventions enables detecting presence and number of certain bacteria among a plurality with high-accuracy due to combined effect applied set of probes.;29 cl, 2 dwg, 7 tbl, 1 ex