CULTIVATED HYBRID ANIMAL CELLS OF MUS MUSCULUS L.-EN-4C9 - PRODUCER OF MONOCLONAL ANTIBODIES AGAINST ENDOGLIN (CD105)

摘要

FIELD: biochemistry.;SUBSTANCE: invention relates to biochemistry. Strain of cultivated hybrid animal cells is disclosed, Mus musculus L. - EN-4C9 – which is producer of monoclonal antibodies against human endoglin (CD105). Strain is created as result of merging of cultivated cells of mouse myeloma SP-2/0 and splenocytes of mice-hybrids F1 (SJL/J x BALB/c), immunized with recombinant human endoglin. Obtained strain EN-4C9 is deposited in Russian collection of cell cultures of vertebrates (Institute of Cytology of RAS) under number RKKK (P) 771D.;EFFECT: invention enables to obtain monoclonal antibodies against human endoglin with wide spectrum of application, in particular for detecting native endoglin on membranes of living and dead cells, as well as for determining concentration of endoglin in human blood serum by ELISA.;1 cl, 5 dwg, 6 tbl, 6 ex