METHOD OF IDENTIFYING RASPBERRY VARIETIES BASED ON RAPD-MARKERS

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to biochemistry, specifically to a method of identifying Agawam, Atlanta, Indian Summer II, the Fugitive, Diamond, Bryansk wonder, Hercules, Yellow Giant, Golden Autumn, Cumberland, Loganberri, Lawton, Orange miracle, Penguin, Sunshine, Tayberri, Tornfri, Elegant raspberry varieties based on RAPD-markers, comprising selection of plant material, extraction of DNA from the plant material, DNA amplification in a reaction mixture with a sequential participation of a few random primers, separation of the amplification products by electrophoresis, visualization of the amplification products under UV light and detection of unique allele combinations of all amplified areas (loci) among the amplification products by all used primers which are specific to this variety of profiles. DNA amplification is carried out with a sequential participation of the following random primers: Oligo 4 - 5'-CAAACGGCAC-3', Oligo 6 - 5'-CACGGCGAGT-3', Oligo 29 - 5'-AAGAGCCCGT-3', Oligo 94 - 5'-GGACGGGTGC-3', Oligo 98 - 5'-GGGTAACGCC-3', Oligo 105 - 5' -AATCGGGCTG-3'.;EFFECT: invention can effectively identify the varieties of raspberry.;4 cl, 1 dwg, 2 tbl, 5 ex