METHOD AND SET FOR DIPHTHERIA DIAGNOSTICS WITH VERIFICATION OF CAUSATIVE PATHOGEN TOXYGEN STRAINS

摘要

FIELD: medicine.;SUBSTANCE: group of inventions concerns a method for diphtheria diagnostics, which involves clinical material taking from a patient, DNA extraction, mixing of the extracted DNA and mixture of No.1 containing a PCR buffer, a mixture of nucleotides (dNTP),MgCl₂, Betaine solution, primers, heating, subsequent cooling, addition of mixture No.2 containing a working dilution of Bst polymerase; isothermal amplification, detection of amplification products under horizontal electrophoresis in an agarose gel by comparing the electrophoretic mobility of the resulting DNA fragments with the mobility of a control DNA sample of Corynebacterium diphtheriae toxigenic strain and diphtheria diagnostics. The group of inventions also concerns a kit for diphtheria diagnostics.;EFFECT: acceleration, simplification of diphtheria diagnostics, high sensitivity and specificity due to simultaneous verification of toxigenic and non-toxigenic pathogen strains with refinement of mechanisms for the absence of diphtheria toxin gene expression.;2 cl, 1 ex