COMPOSITION OF AGAR COATINGS FOR DETERMINING MACHUPO - BOLIVIAN HEMORRHAGIC FEVER VIRUS POPULATION CONCENTRATION AND STRUCTURE WITH USING METHOD OF NEGATIVE COLONIES

摘要

FIELD: pharmacology.;SUBSTANCE: agar coating composition for identifying Machupo virus is proposed. The primary agar coating comprises: an amino acid vitamin complex (AVC), Earle solution, fetal calf serum, 5% sodium bicarbonate solution, demineralized water, 2.9% solution of 1-glutamine, antibiotics, bacto-agar "BacteriologCal" or "Bacto™ Agar ". The secondary agar coating contains: an amino acid vitamin complex (AVC), Earle solution, fetal calf serum, sodium bicarbonate, demineralized water, 0.1% solution of neutral red, a bacto-agar "Bacteriologi Cal" or "Bacto™ Agar".;EFFECT: increasing the sensitivity of the Machupo virus detection method.;3 tbl, 4 ex